概述
Introduction
純羊乳是高端乳制品，脂肪球小、易吸收，適合乳糖不耐受者、老人和嬰幼兒，還富含乳清蛋白、免疫球蛋白及多種營養(yǎng)素，不飽和脂肪酸占比高。

因其產(chǎn)量少、供需缺口大，發(fā)生多起羊乳摻假問題，主要以成本更低的牛乳為摻假成分。針對頻發(fā)羊乳摻假問題，消費者面臨牛乳過敏風(fēng)險，可能引發(fā)皮膚瘙癢、紅腫、惡心嘔吐，甚至導(dǎo)致喉頭水腫、呼吸困難、過敏性休克；企業(yè)會因產(chǎn)品不合格引發(fā)糾紛，造成經(jīng)濟損失、損壞品牌信譽。

青島普瑞邦生物工程有限公司推出PriboStripTM羊乳中牛乳摻假快速檢測試紙條，利用雙抗夾心法原理，靈敏度為1 %，通過檢測牛乳中的α-乳白蛋白初步判定羊乳中是否摻入牛乳，為食品生產(chǎn)及檢測機構(gòu)提供高效可靠的檢測解決方案，助力食品安全。

Pure goat milk is a high-end dairy product. It contains small fat globules and is easily absorbed, making it suitable for people with lactose intolerance, the elderly, and infants. It is also rich in whey protein, immunoglobulins, and various nutrients, with a high proportion of unsaturated fatty acids.
Due to limited goat milk production, a significant gap exists between supply and demand. As a result, goat milk is sometimes adulterated with cow milk to reduce production costs. The frequent occurrence of such adulteration poses health risks to consumers, particularly those allergic to cow milk. Allergic reactions may include skin itching, redness and swelling, nausea, and vomiting, and in severe cases may lead to laryngeal edema, breathing difficulties, or anaphylactic shock. In addition, enterprises may face consumer disputes due to substandard products, resulting in economic losses and damage to brand reputation.

產(chǎn)品原理及優(yōu)勢

Product Principles and Advantages
雙抗夾心法：利用抗α-乳白蛋白單克隆抗體 1（Ab-1）和抗體 2 與膠體金的結(jié)合物（Ab-2-Gdd）結(jié)合抗原的不同表位，從而實現(xiàn)對目標蛋白的定性檢測。

1. 特異性強：采用抗α-乳白蛋白單克隆抗體 1，特異性識別牛乳中α-乳白蛋白，與其他常規(guī)基質(zhì)無交叉反應(yīng)。

2. 靈敏度高：靈敏度為1 %，每百毫升羊奶含1 mL牛奶（稀釋100倍）時可檢出陽性。

3. 操作便捷高效：樣本前處理只需稀釋即可直接檢測，10 min內(nèi)肉眼可直接判讀結(jié)果。

4. 應(yīng)用場景廣泛：可用于羊乳加工廠的原料入廠質(zhì)控、奶站、牧場及經(jīng)銷商的成品入庫檢驗、以及監(jiān)管部門產(chǎn)品品質(zhì)抽查等場景。

Detection Principle

Bispecific antibody sandwich method: A conjugate of anti-α-lactalbumin monoclonal antibody 1 (Ab-1) and colloidal gold-labeled monoclonal antibody 2 (Ab-2-Au) is used to bind different epitopes of the target antigen. Through this specific antigen–antibody reaction, qualitative detection of the target protein is achieved.

Product Features

1）Strong specificity:
Anti-α-lactalbumin monoclonal antibody 1 specifically recognizes α-lactalbumin in cow’s milk and shows no cross-reactivity with other common food matrices.

2）High sensitivity:
The detection sensitivity is 1%. A positive result can be obtained when 1 mL of cow’s milk is present in 100 mL of goat milk (equivalent to a 1:100 dilution).

3）Convenient and efficient operation:
Sample pretreatment requires only simple dilution. Testing can be performed directly, and results can be read by the naked eye within 10 minutes.

4）Application scenarios:
Suitable for raw material quality control in goat milk processing plants, inspection of finished products at milk stations, pastures, and distribution points, as well as product quality spot checks by regulatory authorities.

產(chǎn)品參數(shù)
Product Parameter

 

檢測方法
Method
1. 使用前將試紙條和待檢樣本溶液恢復(fù)至室溫。
2. 將羊奶或羊奶粉用樣本稀釋液稀釋100倍為待測液。
3. 取 200 uL 待測液加入離心管中，將取出的試紙條直接插入離心管中，注意液面高度勿高于箭頭處。10min后即可判讀結(jié)果。

1. Before use, allow the test strip and the sample solution to reach room temperature.

2. Dilute the goat milk or goat milk powder 100-fold with the sample diluent to obtain the test solution.

3. Pipette 200 µL of the test solution into a centrifuge tube. Insert the test strip directly into the tube. Ensure that the liquid level does not exceed the arrow mark on the strip. Interpret the result after 10 minutes.

檢測結(jié)果

Results Analysis

 

1. 當試紙條僅有C線呈色時，為陰性結(jié)果。

2. 當試紙條C線與T線呈色時，為陽性結(jié)果。

3. 當試紙條C線不呈色時，為反應(yīng)失敗。請檢查試紙條外包裝是否破損、試紙條本身是否受潮，或樣本濃度是否過高，并重新取一組試紙條做測試。

1. Negative result: Only the C line of the test strip is colored.

2. Positive result: Both C and T lines are colored.

3. Invalid result: When the C line of the test strip is not colored, the reaction fails. Please check the following possible reasons, and retest with a new test strip:

A: If the test strip packaging is damaged.

B: If the test strip is damp.

C: If the sample is too thick or too concentrated.

過敏原產(chǎn)品系列

Allergen product series

其他牛奶檢測相關(guān)產(chǎn)品如下：

Other milk testing related products are as follows：

 

青島普瑞邦PriboStrip™ 全系列食品過敏原快速檢測平臺提供多種的過敏原檢測產(chǎn)品，助力企業(yè)嚴控過敏原交叉污染風(fēng)險，具體項目如下：

• 八大過敏原類： 花生、大豆、雞蛋、麩質(zhì)、甲殼類、魚類、牛奶、堅果（如杏仁、榛子）等。

• 其他常見過敏原類：芝麻、蕎麥、苦蕎、溶菌酶和組胺等。

• 蔬菜系列：羽扇豆、芹菜等。

• 調(diào)味品系列：芥末。

Qingdao PriboStrip™ offers a full range of rapid food allergen detection platforms, providing a wide variety of allergen detection products to help enterprises effectively control the risk of allergen cross-contamination. The available products include:
• The eight major allergens: peanuts, soybeans, eggs, gluten, crustaceans, fish, milk, nuts (such as almonds, hazelnuts), etc.

• Other common allergens: sesame, buckwheat, bitter buckwheat, lysozyme and histamine, etc.

• Vegetable series: Lupin beans, celery, etc.

• Condiment series: Mustard.