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5089 次 腫瘤外泌體RNA通過(guò)激活肺泡上皮TLR3招募嗜中性粒細(xì)胞促進(jìn)肺預(yù)轉(zhuǎn)移微環(huán)境形成 2016-8-11
腫瘤外泌體RNA通過(guò)激活肺泡上皮TLR3招募嗜中性粒細(xì)胞促進(jìn)肺預(yù)轉(zhuǎn)移微環(huán)境形成研究背景1889年，Stephen Paget通過(guò)對(duì)大量腫瘤的解剖學(xué)研究發(fā)現(xiàn)，特定的腫瘤細(xì)胞總是傾向于轉(zhuǎn)移到特定的組織器官。假
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10661 次 如何有效分離提取cfDNA用于檢測(cè)？ 2016-5-26
說(shuō)起腫瘤、癌癥，已經(jīng)不再像多年前一樣讓談癌色變，但仍舊為人們所恐懼。成功治療腫瘤還是以早發(fā)現(xiàn)早治療為主。那么盡早檢測(cè)和發(fā)現(xiàn)腫瘤便是首當(dāng)其沖的核心。液體活檢(Liquid biopsy)的檢測(cè)方法，
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6875 次 一年4篇Cell一作論文 大牛分享了10條科研經(jīng)驗(yàn) 2016-2-25
羅納德·韋爾博士（Ronald D. Vale）是加州大學(xué)舊金山分校（UCSF）的細(xì)胞與分子藥理學(xué)教授。研究方向是驅(qū)動(dòng)蛋白和動(dòng)力蛋白分子馬達(dá)。他創(chuàng)造了一個(gè)科研界的世界記錄：一年內(nèi)作為第一作者發(fā) 4 篇高
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7923 次 Wheaton細(xì)胞培養(yǎng)系列CELLine生物反應(yīng)器工作原理及特點(diǎn) 2015-5-27
Wheaton的CELLine生物反應(yīng)器采用細(xì)胞室與培養(yǎng)基室半透膜分離培養(yǎng)技術(shù)，突破了傳統(tǒng)細(xì)胞培養(yǎng)的空氣，營(yíng)養(yǎng)物質(zhì)，代謝抑制因子對(duì)細(xì)胞生長(zhǎng)的制約。最大程度的模擬了細(xì)胞在有機(jī)體的生長(zhǎng)環(huán)境。實(shí)現(xiàn)了高
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6481 次 人全血Foxp3實(shí)驗(yàn)步驟 2014-12-3
需要試劑 HumanRegulatoryTCellWholeBloodStainingKit（#88-8996-40） 包含組分： (1)、1XRBCLysisBuffer:200mL (2)、FlowCytometryStainingBuffer:600mL (3)、Fixation/PermeabilizationCon
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3241 次 Cell-Based ELISA簡(jiǎn)介以及應(yīng)用 2014-9-11
1、Cell-Based ELISA 的優(yōu)點(diǎn)： Cell-Based ELISA（基于細(xì)胞的 ELISA）是一種全新的ELISA技術(shù)，有兩個(gè)最突出的優(yōu)點(diǎn)：1.1不需要抽提蛋白、包被微孔板：細(xì)胞直接在微孔板里培養(yǎng)，待檢測(cè)的時(shí)候，將細(xì)
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10376 次 血細(xì)胞形態(tài)學(xué)檢查的重要意義 2014-6-28
血液一般檢驗(yàn)取材容易、檢測(cè)便捷，仍然是篩檢疾病、遴選其他實(shí)驗(yàn)檢查的首要程序。血細(xì)胞計(jì)數(shù)及血液形態(tài)學(xué)檢查是血液檢驗(yàn)項(xiàng)目中最基礎(chǔ)、最常用的檢驗(yàn)。血細(xì)胞形態(tài)學(xué)檢驗(yàn)是檢驗(yàn)醫(yī)學(xué)的基礎(chǔ)和核心，
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5791 次 T Cell Receptor 信號(hào)通路圖 2012-7-23
The T Cell Receptor plays a key role in the immune system. The specificity of the receptor is governed by the binding site formed from the mature alpha and beta chains (shown here)
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4676 次 B Cell Receptor 信號(hào)通路圖 2012-7-13
B cells produce immunoglobulins (Ig, antibodies) that specifically bind antigen molecules. B cells first produce a membrane-bound form of immunoglobulin, the B cell receptor, as pa
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2546 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
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1777 次 Fluorescent Staining of Cells 2012-6-20
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS, solution A. Procedure:1. Fix a
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3459 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
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3311 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
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3026 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
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1907 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
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2144 次 Isolation of lymphatic endothelial cells 2012-3-12
Dermal Cell Suspensions1.Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2.Dermal sheets were prepared by incubatio
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5793 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
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3425 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
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2461 次 Placental trophoblast and chorionic cell cultures 2011-12-16
Placental trophoblast and chorionic cell cultures 1.Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2.Term human placentae a
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4035 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut
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