MED64應(yīng)用手冊(cè)：人類(lèi)干細(xì)胞來(lái)源的心肌細(xì)胞應(yīng)用

關(guān)鍵詞：
人類(lèi)干細(xì)胞來(lái)源的心肌細(xì)胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏動(dòng)頻率 beat frequency; 場(chǎng)電位時(shí)程 field potential duration (FPD)
慢性藥物作用 chronotropic drug effects; 藥物篩選 drug screening

人類(lèi)干細(xì)胞來(lái)源的心肌細(xì)胞是制藥公司和科研機(jī)構(gòu)在研究中應(yīng)用得比較多的樣本。由于人類(lèi)干細(xì)胞來(lái)源的心肌細(xì)胞具有和原代人類(lèi)心肌細(xì)胞一樣的遺傳背景，它們的藥理學(xué)特性應(yīng)該非常相似，因此結(jié)合人類(lèi)干細(xì)胞來(lái)源的心肌細(xì)胞與MED64多電極胞外記錄系統(tǒng)的平臺(tái)已成為了研究人類(lèi)遺傳性心肌疾病模型和藥物篩選的主流。
只需要將hSC-CMs培養(yǎng)在MED記錄電極上，就可以從MED64系統(tǒng)中記錄和提取出基本的電生理參數(shù)，比如搏動(dòng)頻率、以場(chǎng)電位時(shí)程等。另外，由于電極的非侵入特性，穩(wěn)定的記錄可以持續(xù)非常長(zhǎng)的時(shí)間，因此慢性藥物的效果也能夠在MED64系統(tǒng)上進(jìn)行測(cè)量。不僅如此，MED64還可以使用任意電極輸出刺激來(lái)Pace hSC-CMs，因此可用來(lái)研究頻率依賴(lài)或使用依賴(lài)的藥物作用。

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